Characterization of the Immunotoxicity of Chlorothalonil to Striped Bass Phagocytes Following in vitro Exposure

摘要

The fungicide chorothalonil (TCIN), with both agricultural and horticultural applications, is a common aquatic pollutant in the Chesapeake Bay and its tributaries. The immunotoxic potential of TCIN was investigated following 20h in vitro exposure of striped bass (Morone saxatilus) macrophages to a range of sublethal concentrations. Cells from the anterior kidney were separated by density-gradient centrifugation to obtain phagocytes. Following TCIn exposure, reactive oxygen species (ROS) production was evaluated using luminol- augmented chemiluminescence; phagocytic capacity was measured by ingestion of FITC-conjugated yeast; and NADPH production was estimated by monitoring the reduction of a water-soluble tetrazolium, MTS.