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High-Throughput Screening of Silver Nanoparticle Stability and Bacterial Inacthration in Aquatic Media: Influence of Specific Ions

机译:高通量筛选银纳米颗粒的稳定性和水生介质中细菌的不准确性:特定离子的影响

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摘要

Although silver nanoparticles are being exploited widely in antimicrobial applications, the mechanisms underlying silver nanoparticle antimicrobial properties in environmentally relevant media are not fully understood. The latter point is critical for understanding potential environmental impacts of silver nanoparticles. The aim of this study was to elucidate the influence of inorganic aquatic chemistry on silver nanoparticle stability (aggregation, dissolution, repreciprtation) and bacterial viability. A synthetic "fresh water" matrix was prepared comprising various combinations of cations and anions while maintaining a fixed ionic strength. Aggregation and dissolution of silver nanoparticles was influenced by electrolyte composition; experimentally determined ionic silver concentrations were about half that predicted from a thermodynamic model and about 1000 times lower than the maximum dispersed silver nanoparticle concentration. Antibacterial activity of silver nanoparticles was much lower than Ag ions when compared on the basis of total mass added; however, the actual concentrations of dissolved silver were the same regardless of how silverwas introduced. Bacterial inactivation also depended on bacteria cell type (Gram-posrbveegative) as well as the hardness and alkalinity of the suspending media. These simple, but systematic studies-enabled by high-throughput screening-reveal the inherent complexity associated with understanding silver nanoparticle antibacterial efficacy as well as potential environmental impacts of silver nanoparticles.
机译:尽管银纳米颗粒在抗菌应用中得到了广泛的利用,但对与环境有关的介质中银纳米颗粒抗菌特性的潜在机理尚未完全了解。后一点对于理解银纳米颗粒的潜在环境影响至关重要。这项研究的目的是阐明无机水化学对银纳米颗粒稳定性(聚集,溶解,再沉淀)和细菌生存力的影响。制备包含阳离子和阴离子的各种组合同时保持固定的离子强度的合成“淡水”基质。电解质组成影响银纳米颗粒的聚集和溶解。实验确定的离子银浓度约为热力学模型预测值的一半,比最大分散银纳米颗粒浓度低约1000倍。基于总添加量,银纳米颗粒的抗菌活性远低于银离子。但是,无论如何引入银,溶解银的实际浓度都相同。细菌失活还取决于细菌细胞类型(革兰氏阳性/阴性),以及悬浮介质的硬度和碱度。这些简单但系统的研究通过高通量筛选得以实现,揭示了与了解银纳米颗粒的抗菌功效以及银纳米颗粒的潜在环境影响相关的固有复杂性。

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  • 来源
    《Environmental Science & Technology》 |2010年第19期|p.7321-7328|共8页
  • 作者单位

    Department of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

    rnDepartment of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

    rnDepartment of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

    rnDepartment of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

    rnDepartment of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

    rnDepartment of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

    rnCalifornia NanoSystems Institute University of California, Los Angeles,California, 90095 Molecular Screening Shared Resource, University of California, Los Angeles,California, 90095;

    rnDepartment of Civil and Environmental Engineering University of California, Los Angeles,California, 90095 California NanoSystems Institute University of California, Los Angeles,California, 90095;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 入库时间 2022-08-17 14:04:01

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