Investigation on the interaction differences of algogenic dissolved organic matter released by Prorocentrum donghaiense with phenanthrene and 3-methylphenanthrene using spectroscopy

摘要

Despite the high algogenic dissolved organic matter (A-DOM) content in the marine environment, the impact of A-DOM on the environmental behavior of alkylated polycyclic aromatic hydrocarbons (A-PAHs) has rarely been reported. Thus, this study sought to investigate the changes in A-DOM and its interaction with phenanthrene (Phe) and 3-methylphenanthrene (3-MPhe). The results showed that the spectroscopic characteristics of A-DOM changed dynamically during the entire growth period of Prorocentrum donghaiense. The total proportions of humic-like components in A-DOM, including marine humic-like and longer wavelength humic-like components, increased from 26.30% at the initial phase to 40.42% at the decline phase, but those of protein-like components decreased. Humic-like components showed stronger interaction capabilities with Phe/3-MPhe than that of protein-like components. The interaction characteristics and increased the humic-like components content indicated their prominent role in the interaction of A-DOM and Phe/3-MPhe. 3-MPhe had stronger hydrophobic interactions with the humiclike components and the methyl group increased pi -pi interactions with A-DOM on day 28. Methyl group also increased the molecular weight and steric hindrance of 3-MPhe, resulting in a lower interaction capability with A-DOM than that of Phe on day 16. The diverse interaction behaviors of A-DOM fluorescent components with Phe and 3-MPhe were largely attributed to dynamic changes in the A-DOM characteristics. Methyl substitution varied the hydrophobicity and molecular weight of Phe and 3-MPhe, and further influenced the interactions with A-DOM fluorescent components. These results highlight the vital impact of dynamic A-DOM on the environmental behavior of PAHs, and the differences in the environmental behavior between parent and alkylated PAHs should also receive additional attention. (C) 2020 Elsevier Ltd. All rights reserved.