Identification and quantitative confirmation of dinitropyrenes and 3-nitrobenzanthrone as major mutagens in contaminated sediments

摘要

Polar fractions of a sediment extract of the industrial area of Bitterfeld, Germany, have been subjected ft effect-directed identification of mutagens using the Ames fluctuation assay with TA98. Mutagenicity coul be well recovered in several secondary and tertiary fractions. Dinitropyrenes and 3-nitrobenzanthrone coul be confirmed to contribute great shares of the observed mutagenicity. In addition, a multitude of polar polycycl aromatic compounds has been tentatively identified in mutagenic fractions including nitro-PAHs, azaarene ketones, quinones, hydroxy-compounds, lactones and carboxylic acids although their contribution to mut, genicity could not be quantified due to a lack of standards. Diagnostic Salmonella strains YG1024 and YG1CK were applied to confirm the contribution of nitro-aromatic compounds. We suggest the inclusion of dinitropyrem and 3-nitrobenzanthrone into sediment monitoring in order to minimize the mutagenic risk to aquatic organisn and to human health.