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首页> 外文期刊>Journal of Virology >Endonuclease R-EcoRII restriction of bacteriophage f1 DNA in vitro: ordering of genes V and VII, location of an RNA promotor for gene VIII.
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Endonuclease R-EcoRII restriction of bacteriophage f1 DNA in vitro: ordering of genes V and VII, location of an RNA promotor for gene VIII.

机译:内核酸酶R-EcoRII在体外噬菌体F1 DNA的限制:基因V和VII的排序,基因VIII的RNA促进剂的位置。

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摘要

Replicative form DNA of bacteriophage f1 was found to be sensitive in vitro to restriction by endonuclease R-EcoRII if the DNA was isolated from an Escherichia coli strain deficient in cytosine methylase activity. A similar observation was previously made with DNA from the closely related bacteriophage fd (S. Schlagman, S. Hattman, M. S. May, and L. Berger, submitted for publication). The two DNA fragments produced by the endo R-EcoRII digestion of f1 DNA were localized on the f1 cleavage map and their genetic content was determined. The polypeptides synthesized in a "coupled" transcription-translation system under the direction of each RII fragment were examined. The results of such experiments allow the ordering of genes V and VII and indicate the location of a RNA promotor for gene VIII.
机译:如果DNA从胞嘧啶甲酶活性缺乏的大肠杆菌菌株中分离,发现噬菌体F1的复制形式的噬菌体F1的DNA对核核酸酶R-ECORII的限制敏感。预先用密切相关的噬菌体FD与DNA制备了类似的观察结果(S. Schlagman,S. Hattman,M. S. May.和L. Berger,提交出版物)。通过Endo R-EcoRII消化F1 DNA产生的两个DNA片段局部化在F1切割图上,并测定它们的遗传含量。检查在每个RII片段的方向下合成的在“偶联的”转录翻译系统中合成的多肽。此类实验的结果允许v和vii的排序,并表明基因VIII的RNA促进剂的位置。

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