Quantification of Sugar Compounds and Uronic Acids in Enzymatic Hydrolysates of Lignocellulose Using High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection

摘要

A method was developed for simultaneous determination of seven sugar compounds and two uronic acids by high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) using a CarboPac PA20 column under a gradient elution (mobile phase: H_2O/200 mM NaOH/100 mM NaAc) at 0.4 mL/min. Under these conditions, D-(-)-arabinose, D-(+)-galactose, D-(+)-glucose, D-(+)-mannose, D-(+)-xylose, D-(-)-fructose, D-(+)-cellobiose, D-galacturonic acid, and D-glucuronic acid were all separated in 46 min. The calibration curve, detection limits, reproducibility and accuracy were also determined. Furthermore, seven sugar compounds and two uronic acids were all detected in the enzymatic hydrolysates of different lignocellulosic materials treated by different pretreatment processes. Our results demonstrate that this method was feasible for sugar compounds separation without the need for derivatization and organic solvent, and it also has the advantages of having low interference, high sensitivity, low detection limit of picomol, and excellent reproducibility and accuracy.