Blocking type anti-TSH receptor antibodies detected by radioreceptor assay in graves' disease

摘要

We previously developed a radioreceptor assay which is presumably specific for detection of blocking-type anti-TSH receptor(TSHR)antibodies using unsolubilized porcine TSHR. Employing this assay, we measured blocking TSH-binding inhibitor immunoglobulin(TBII)in the sera from 30 untreated Graves' patients and com- pared the results with those of a bioassay measuring thyroid stimulation blocking antibodies(TSBAb). Blocking TBII was positive in 9 of 3 sera(30/100)and the blocking TBII activity was correlated with the total TBII value, which Was measured by the radioreceptor assay using solubilized porcine TSHR.