Microwave-assisted preparation of biodegradable, hemocompatible, and antimicrobial neem gum-grafted poly (acrylamide) hydrogel using (3)2 factorial design

摘要

The objective of the study was to prepare neem gum polysaccharide graft copolymers of acrylamide (NGP-g-Am) using 3 factorial design. Prepared NGP-g-Am's hydrogels were characterized using UV-visible spectroscopy, FTIR spectral analysis, SEM images, contact angle determination, biodegradability, hemocompatibility, and pH-dependent swelling ability. NGP-g-Am showed more swelling index in all the media like double distilled water, 1 N NaOH, and 0.1 N HC1 than native form. Data obtained through soil burial biodegradation studies were showed t_(90%) for neem gum polysaccharide (NGP) and NGP-g-Am (Nl), 9 and 28 days, respectively. Findings of the Lee-White test for blood clotting time showed the longest clotting time (15.39 ± 0.53 min) for NGP-g-Am (N5) as compared with that for the uncoated glass surface (2.05 ± 0.93 min). Thrombus formed during studies were found to be significantly more in case of uncoated glass surface as compared with N (0.47 ± 0.23 mg), Nl (0.29 ± 0.08 mg), N2 (0.30 ± 0.13 mg), N5 (0.29 ± 0.11 mg), N7 (0.29 ± 0.07 mg), and N9 (0.28 ±0.13 mg). Structure-based docking studies predict that binding of ligands to TLR-4 receptors is significantly more responsible for the antimicrobial effect of both NGP and NGP-g-Am.