Effect of selenium antagonist lead-induced damage on Thl/Th2 imbalance in the peripheral blood lymphocytes of chickens

摘要

Lead (Pb) is a type of toxic metal that can hurt the immune system. Selenium (Se) can reduce the damage caused by heavy metals. To investigate the effects of Se against Pb on bird immune cells, as well as the immunotoxin mechanism of Pb, Se supplementation and/or Pb poisoning chicken models were established. One hundred eighty 1-year-old broiler chickens were randomly divided into four groups (n = 6). The four groups were the control group, the selenium-rich group (Se group), the Pb supplementation group (Pb group) and the Se and Pb compound group (Se + Pb group). The peripheral blood lymphocytes of chickens were collected to test the selenoproteins and cytokine mRNA levels at 30 and 60 d. Determination of the content of Se and Pb in the serum, principal component analysis and ingenuity pathway analysis were performed at the two time points. As a result, Pb exposure increased the content of Pb, activating the Th1/Th2 pathway in peripheral blood lymphocytes. Additionally, this experiment showed that Se supplementation and Pb exposure could influence the mRNA levels of selenoproteins and cytokines in the peripheral blood lymphocytes of chickens. However, all of the parameters that we detected in the experiment indicated that Se supplementation could alleviate the increase of selenoproteins and cytokine mRNA levels and the Th1/Th2 imbalance induced by Pb in peripheral blood lymphocytes. In summary, Se can alleviate the toxic effects caused by Pb in the peripheral blood lymphocytes of chickens, suggesting the antagonism between Se and Pb.