A Study of Recombinant Human Lactoferrin Secreted in Milk of Transgenic Mice

摘要

In this study, the properties of recombinant human lactoferrin isolated from milk of transgenic mice and natural human lactoferrin obtained isolated from breast milk were compared. Identity of both proteins was confirmed using by electrophoretic, immunological, and chromatographic methods. The lactoferrin from milk of transgenic mice exhibited the properties characteristic of human lactoferrin: it effectively chelated iron ions and binds to DNA, heparin, and bacterial lipopolysac-charide. Both proteins displayed identical bactericidal and fungicidal activities. The data obtained in this study allow the created genetic constructs to be used for obtaining producers of human lactoferrin on the basis of farm animals.