Bradykinin Directly Triggers GLUT4 Translocation Via an insulin-Independent Pathway

摘要

Physical exercise induces translocation of GLUT4 from an intracellular pool to the cell surface in skeletal mus- cles and increase glucose uptake via an insulin-inde- pendent pathway. However, the molecular mechanism remains to be identified. Some studies have suggested that bradykinin is locally released form contracting muscles and may be responsible for GLUT4 translocation and the increase of glucose transport in skeletal mus- cles. To determine whether bradykinin directly triggers GLUT4 translocation, we estalbished L6 myotubes, 3T3- L1 adipocytes, and chinese hamster ovary cells stably Expressing c-myc epitope-tagged GLUT4 (GLUT4myc) And bradykinin B_2 receptors.