Activation of acetyl-CoA carboxylase by a glutamate- and magnesium-sensitive protein phosphatase in the isletβ-cell

摘要

Acetyl-CoA carboxylase(ACC)catalyzes the formation of malonyl-CoA, a precursor in the biosynthesis of long- chain fatty acids, which have been implicated in phys- iological insulin secretion. The catalytic function of ACC is regulated by phosphorylation(inactive)-de- Phosphorylation(active). In this study we investigated Whether similar regulatory mechanisms exist for ACC in The pancreatic isletβ-cell. ACC was quantitated in nor- Mal rat islets, human islets, and clonalβ-cells(HIT-15 Or INS-1)using a[~14C]bicarbonate fixation assay.