Regulatory Effects of Berberine on Adipogenic Differentiation in Porcine Intramuscular Preadipocytes Through the Multi-Gene Pattern

摘要

Background and Objective: Berberine is well known as a traditional anti-bacterial and anti-inflammatory Chinese medicine. Recently, it was reported to have interesting effects on adipogenic differentiation, glucose uptake, insulin resistance , etc. However, the molecular mechanisms of these underlying pharmacological effects are not sufficiently elucidated. Here, the mechanism of berberine on adipogenic differentiation in porcine intramuscular preadipocytes was investigated. Materials and Methods: Preadipocytes were obtained for primary culture from the healthy piglets under sterile conditions. Preadipocytes viability was detected by MTT assay. Intracellular lipid droplets were observed under microscopy by oil-red O staining and the extraction quantified by colorimetry at room temperature. The key adipogenic genes were detected by real-time quantitative PCR. Luciferase assays were used to validate the targeting relationship between microRNA and the MAPK signalling pathway. Results: When porcine preadipocytes were induced into adipogenic differentiation, cell proliferation and accumulation of intracellular lipid droplets were decreased in a time- and dose-dependent manner by berberine. Berberine attenuated adipogenic differentiation by impairing some key adipogenic genes expression, such as PPAR γ and adiponectin and even provoking comprehensive effects through the interference with the interaction of a microRNA (miR-143) and certain a target gene ( MAP2K5 ). Conclusion: The inhibiting effects of berberine on adipogenic differentiation in porcine intramuscular preadipocytes are through the multi-gene pattern, which provides novel evidence for treatments of berberine to gain healthy animal-based foods against excessive fatty deposits, even human obesity, hyperlipidemia, diabetes, etc.