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Evaluation of Quadruple Real-Time PCR Method to Detect Enterococci Carrying Vancomycin-Resistant Genes vanA, vanB, vanM in Rectal Swabs

机译:评价二次实时PCR方法检测肠球菌携带万古霉素抗性基因的肠球菌,VANB,vanm在直肠拭子

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Purpose: To evaluate the sensitivity and accuracy of the quadruple real-time PCR method for the detection of enterococci carrying vancomycin-resistant genes vanA, vanB , and vanM in rectal swabs. Methods: Choosing PCR-sequenced DNA extracted directly from rectal swabs as the gold standard, the results of the quadruple real-time PCR method and the traditional method (screening culture combined PCR-sequencing method whose DNA extracted from single colony) were compared with the gold standard. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the quadruple real-time PCR method and the traditional method were obtained. The time required for the three methods was calculated. Results: The results between gold standard and the quadruple real-time PCR method were similar. Compared to the traditional method, the quadruple real-time PCR method had a much higher sensitivity, specificity, PPV, NPV, and consistency. Our study found that the quadruple real-time PCR method is beneficial for detection of enterococci carrying vanM with vancomycin heteroresistance. The traditional method had high specificity and NPV, but its sensitivity and PPV were not ideal. The time needed for gold standard is a minimum of 28 h; the quadruple real-time PCR method takes 2–3 h while the traditional method consumes a minimum of 72 h. Conclusion: The quadruple real-time PCR method can provide a rapid and reliable result for the diagnosis of patients with colonized vancomycin-resistant enterococci. This new method is beneficial for the active screening, timely clinical treatment measures, epidemiological research, and hospital monitoring of the enterococci carrying vancomycin-resistant gene, especially for the enterococci with vancomycin heteroresistance carrying vanM .
机译:目的:评估四重实时PCR方法的敏感性和准确性,用于检测肠球菌耐肠杆菌素,VANB和VANM在直肠拭子中的肠球菌。方法:选择直接从直肠拭子提取的PCR测序DNA作为金标准,与备份相比黄金标准。获得了四重实时PCR方法和传统方法的敏感性,特异性,阳性预测值(PPV),负预测值(NPV)和准确性。计算三种方法所需的时间。结果:黄金标准与四重实时PCR方法之间的结果相似。与传统方法相比,四重实时PCR方法具有更高的敏感性,特异性,PPV,NPV和一致性。我们的研究发现,二次实时PCR方法有利于检测携带Vancomycin杂项的肠球菌。传统方法具有高特异性和NPV,但其敏感性和PPV并不理想。黄金标准所需的时间至少为28小时;四重实时PCR方法需要2-3小时,而传统方法消耗至少72小时。结论:四重实时PCR方法可以为患有殖民化万古霉素肠内肠球菌的诊断提供快速可靠的结果。这种新方法有利于主动筛选,及时的临床治疗措施,流行病学研究和肠球菌抗性基因的医院监测,特别是对于携带VanM的万古霉素杂项肠球菌。

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