The complete chloroplast genome of an endemic plant to Korea, Cardamine amaraeformis Nakai.: genome structure and phylogenetic analysis

摘要

The plant Cardamine amaraeformis Nakai. is considered an endemic plant to Korea. However, due to the similar morphological characteristics of C . amaraeformis with C . scutata , it is not easy to distinguish these two species. Here, the complete chloroplast genome of C . amaraeformis was sequenced and characterized. The chloroplast genome of C . amaraeformis was 155,598?bp in length, comprising a large single-copy (LSC) region of 84,574?bp and a small single-copy (SSC) region of 17,976?bp and a pair of inverted repeats (IRs) of 26,524?bp. The genome contained 131 genes, including 86 protein-coding (PCGs), 37 tRNA and 8 rRNA genes. Of those, 6 PCGs, 8 tRNA and 4 rRNA genes were duplicated in the IR region and one gene was a pseudogene. The GC content of the C . amaraeformis chloroplast genome was 36.3%. Phylogenetic analysis revealed that all Cardamine species formed a monophyletic clade and C . amaraeformis was closely associated with C . parviflora . Therefore, the present study could help to distinguish C . scutata and resolve the phylogenetic relationships among the Cardamine lineage.