UNIVERSAL VECTOR FOR STABLE TRANSFORMATION OF CHLOROPLAST GENOME, METHOD FOR STABLE TRANSFORMATION OF PLANT-TARGET, METHOD FOR CONFERRING TO PLANT RESISTANCE TO PLANT-TARGET AGAINST HERBICIDE, METHOD FOR ASSAY OF TRANSFORMATION OF CHLOROPLAST AND STABLY TRANSFORMED CHLOROPLAST GENOME

摘要

FIELD: genetic engineering of plants, agriculture.;SUBSTANCE: vector involves the expression cassette comprising a sequence encoding the interesting peptide that is joined with regulatory sequences providing expressing of the encoding sequence in chloroplasts of plant-target. Cassette-flanking sequences are homologous with conservative spacer sequences of chloroplast genome of target that provides the stable integration of encoding sequence into chloroplast genome. Insertion of mentioned vector into genome of plants of different species allows conferring to them new properties that are stably inherited in progeny.;EFFECT: valuable biological properties of vector, improved transformation and assay.;29 cl, 62 dwg, 2 tbl, 16 ex