A STABLE CHLOROPLAST TRANSFORMATION AND EXPRESSION VECTOR

摘要

A stable chloroplast transformation and expression vector which is capable of introducing multiple genes into a selected plant by a single integration event, said vector comprising as operably linked components, in the 5' to the 3' direction of translation, a promotor operative in said plastids which drives a multi-gene operon codes for a biopharmaceutical protein, said multi-gene operon comprising a first ORF encoding a first protein and a second ORF encoding a second protein, wherein transcription of said multi-gene operon generates a polycistron encoding said first protein and said second protein, wherein at least one of said proteins is a biopharmaceutical protein.