首页> 外文期刊>Microbiome >The administration of intranasal live attenuated influenza vaccine induces changes in the nasal microbiota and nasal epithelium gene expression profiles
【24h】

The administration of intranasal live attenuated influenza vaccine induces changes in the nasal microbiota and nasal epithelium gene expression profiles

机译:鼻内活病毒的施用疫苗诱导鼻腔微生物和鼻上皮基因表达谱的变化

获取原文
获取外文期刊封面目录资料

摘要

Viral infections such as influenza have been shown to predispose hosts to increased colonization of the respiratory tract by pathogenic bacteria and secondary bacterial pneumonia. To examine how viral infections and host antiviral immune responses alter the upper respiratory microbiota, we analyzed nasal bacterial composition by 16S ribosomal RNA (rRNA) gene sequencing in healthy adults at baseline and at 1 to 2 weeks and 4 to 6 weeks following instillation of live attenuated influenza vaccine or intranasal sterile saline. A subset of these samples was submitted for microarray host gene expression profiling. We found that live attenuated influenza vaccination led to significant changes in microbial community structure, diversity, and core taxonomic membership as well as increases in the relative abundances of Staphylococcus and Bacteroides genera (both p??0.05). Hypergeometric testing for the enrichment of gene ontology terms in the vaccinated group reflected a robust up-regulation of type I and type II interferon-stimulated genes in the vaccinated group relative to controls. Translational murine studies showed that poly I:C administration did in fact permit greater nasal Staphylococcus aureus persistence, a response absent in interferon alpha/beta receptor deficient mice. Collectively, our findings demonstrate that although the human nasal bacterial community is heterogeneous and typically individually robust, activation of a type I interferon (IFN)-mediated antiviral response may foster the disproportionate emergence of potentially pathogenic species such as S. aureus. This study was registered with Clinicaltrials.gov on 11/3/15, NCT02597647 .
机译:已显示病毒感染,例如流感,以促进宿主通过致病性细菌和继发性细菌肺炎增加呼吸道的殖民。检查病毒感染和宿主抗病毒免疫应答如何改变上呼吸道微生物群,我们在基线的健康成人中分析了16S核糖体RNA(RRNA)基因测序的鼻细菌组合物,在滴注时在1至2周和4至6周后进行分析减毒流感疫苗或鼻内无菌盐水。这些样品的子集被提交用于微阵列宿主基因表达分析。我们发现现场减毒的流感疫苗接种导致微生物群落结构,多样性和核心分类会员的显着变化,以及葡萄球菌和菌株的相对丰富的增加(P?<0.05)。在疫苗基团中富集基因本体术语的富集性测试反映了疫苗基团中I型和II型干扰素刺激基因的鲁棒上调。翻译小鼠研究表明,多种I:C授权实际上允许更大的鼻腔葡萄球菌持续性,在干扰素α/β受体缺陷小鼠中缺席的反应。统称,我们的研究结果表明,尽管人鼻细菌群落是异质的并且通常是单独稳健的,但是I型干扰素(IFN)介导的抗病毒反应的激活可以培养潜在的致病性物种如S. aureus的不成比例的出现。本研究于NCT02597647于11/3/3/15,在ClinicalTrials.gov注册。

著录项

获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号