Immobilization ofAspergillus Oryzae β-Galactosidase on Newly Prepared Porous Poly(GMA-ST)

摘要

The macroporous and reactive carriers polyGMA-ST was synthesized simultaneously with a mixture of cyclohexanol and lauryl alcohol as liquid pore-forming agents and nano-calcium carbonate as solid one by bulk copolymerization. After the polymer was smashed, particles with diameters ranging 0.15 mm to 0.30 mm were taken as the carrier and the Scanning electron microscopy (SEM) micrographs were done to characterize its surface structure. Under the optimum conditions,β-galactosidaseAspergillus oryzaewas immobilized on the supporter obtained above, its enzyme activity could reach to 535.11U/g dry carrier and the activity recovery of the immobilizedβ-galactosidase was 79.63%. Meanwhile, the basic property and the kinetic data of the immobilized enzyme were determined and compared with those of the free enzyme and satisfactory results were obtained in pH stability, thermal stability and operational stability. The conclusion obtained here indicated that the ploy(GMA-co-ST) prepared concurrently with liquid and solid porogen was suitable to immobilize enzyme.