Immobilization of ?-galactosidase from Aspergillus Oryzae on Macroporous PloyGMA Newly Prepared

摘要

The reactive, macroporous and massive epoxy group-containing polyGMA was synthesized simultaneously with methanol aqueous solution and nano-calcium carbonate as porogen by bulk copolymerization. After the polymer was smashed, particles with diameters in the range of 0.30 to 0.45mm were taken as the carrier and the Scanning electron microscopy (SEM) micrographs were done to characterize its surface structure. Under the optimum conditions, ?-galactosidase was immobilized on the supporter obtained above, the basic property and the kinetic data of all the immobilized enzyme were determined, and satisfactory results were obtained in enzyme activity, activity yield, pH stability, thermal stability, operational stability, and Michaelis constants Km. The conclusion obtained indicated that the ployGMA prepared concurrently with liquid and solid porogen was more suitable to immobilize enzyme than that purely with liquid solution as pore-forming agent.