The optimization method for synthesis of technetium-99m-luteolin as radiotracer in the development of cancer drugs from flavonoid

摘要

The aim of this study is to find the optimum conditions of labeling luteolin flavonoid compounds with technetium-99m (sup99m/supTc) to meet the purity requirements stated in the United States Pharmacopeia. This compound is expected to be a potential radiotracer compound for diagnosing cancer. The optimization method in labeling luteolin with technetium determines the parameters such as pH, SnClsub2/sub.2Hsub2/subO, genistein concentration, and incubation time. Optimization results of Technetium-99m-luteolin labeling obtained optimum pH conditions 8, the amount of SnClsub2/sub.2Hsub2/subO as a reducing agent 60 μL, the optimum amount of luteolin 6 mg/ml, and the optimum incubation time is 30 min. This optimum condition obtained asup99m/supTc-Luteolin radiochemical purity yield of 94.15%. The radiochemical purity percentage of thesup99m/supTc-Luteolin compound has fulfilled the requirements listed at United States Pharmacopeia, which is ≥90%.