Genetic diversity of the dye-producing Javanese Tom Indigofera tinctoria L. from three Indonesian islands based on simple sequence repeat markers.

摘要

Javanese Tom ( Indigofera tinctoria L . ) is commonly used as blue dyes agent by Indonesian fabrics and batik crafters. The research was aimed to identify the genetic diversity and population structure of I. tinctoria L . based on the SSR markers. The research was conducted in 33 regions in three Indonesian islands, with 63 samples. The results from electrophoresis of 15 SSR primers produced 76 bands with 250–750 bp in size. From dendrogram obtained likelihood coefficient of 68–100%. Weirklei3 and Weirklei4 accessions had the highest likelihood coefficient with 100% (same). The highest diversity was recorded in Cirebonean population. The populations from Sumenep, Cirebon, and Flores were grouped as one with high like hood coefficient. Populations from Java, Madura, and Flores islands have high likelihood coefficients. Based on SSR markers, I. tinctoria populations from three Indonesian islands were having a high likelihood coefficient, thus unqualified to be categorized as the infra-species.