MOLECULAR CHARACTERISATION AND EXPRESSION ANALYSIS OF ACC OXIDASE GENE FROM GUZMANIA RUIZ & PAV.

摘要

ACC oxidase is the last key enzyme of ethylene synthesis pathway, while ethylene is a key factor affecting flowering inornamental bromeliad. To understand ACC oxidase gene’s characteristics and its effect on ornamental bromeliad flowering,we cloned 1504bp full-length cDNA sequence (GenBank: JX972145) and 2546bp corresponding genomic sequence(GenBank: JX972146)of GoACO1 (ACC oxidase gene) from Guzmania variety: Ostara. Prokaryotic expression studyshowed that expression of GoACO1 can produced a 41 KD protein precipitation in Escherichia coli DE3(BL-21); Real-timequantitative analysis showed that GoACO1 can express in all tested tissues including floral organ, bract, leaf and scape, andexpression quantity in bract was the highest. Through constructing plant overexpression vector, transforming intoArabidopsis thaliana, and investigating blossom character of T2 generation seeds, we found that first flowering time of thegoal Arabidopsis thaliana was 1.5 days earlier, and their peak flowering time(the number of flowering more than 50%) was1.8 days earlier, compared with wild type one. Taken together, our results suggested that GoACO1can express in all kinds oftissues and seems to promote Arabidopsis thaliana flowering earlier.