Molecular cloning of two 1-aminocyclopropane-1-carboxylate (ACC) synthases and an ACC oxidase from Pelargonium x hortorum cv. Snow Mass leaves.

摘要

Two geranium (Pelargonium x hortorum cv. Snow Mass) 1-aminocyclopropane-1-carboxylate synthase (ACCS) cDNAs (GAC-1 and GAC-2) and an ACC oxidase (ACCO) cDNA (GEFE-1) were identified and characterized. GAC-1 is 1,934 bp long with a 79 bp leader sequence, a 409 bp trailing sequence, and a 1,446 bp open reading frame. GAC-2 is a 1,170 bp long ACCS PCR fragment encoding 390 amino acids. GEFE-1 is 1,291 bp long with a 88 bp leader sequence, a 249 bp trailing sequence, and a 954 bp open reading frame. These cDNAs were used as probes for Northern analysis of different geranium plant parts (leaves, florets, and roots), cuttings stored at 4