DIFFERENTIAL PROTEOMIC ANALYSIS OF SALT STRESS RESPONSE IN JUTE (CORCHORUS CAPSULARIS & OLITORIUS L.) SEEDLING ROOTS

摘要

Jute (Corchorus capsularis & olitorius L.) is mostly grown in Southeast Asian countries and has been recentlysuggested as a promising candidate for planting in wetland and saline soils in China. To effectively breed more salt-tolerantjute cultivars, it is necessary to understand its salt stress-responsive mechanism at molecular level. Morphological,physiological and proteomic analyses were performed on seedlings of two jute genotypes exposed to 50, 100 and 150 mMNaCl, respectively, for four days. Our results indicated that genotype 9511, with lower degree of average index of salt harm(AISH) in leaf, less fallen leaf number/ten plants and higher root proline (Pro) content, was more salt tolerant than genotypeMengyuan. Two-dimensional gel electrophoresis (2-DE) showed that expressions of 44 protein spots were significantlychanged in the seedling roots of the two genotypes in response to salt stress. Thirty-nine (39) differentially expressedproteins were identified by MALDI-TOF-TOF MS, and classified into nine groups. Based on most of the 39 identified saltresponsiveproteins, a salt stress-responsive protein network in jute seedling roots was proposed. After the persistent (for 4d) salt stress, jute seedling would adapt to salt stress through altering signal transduction, accelerating ROS scavenging,impairing energy metabolism, enhancing nucleotide metabolism, lipid metabolism and cell wall metabolism, as well asaltering cytoskeleton in roots. NaCl-responsive protein data will provide insights into salt stress responses and for furtherdissection of salt tolerance mechanisms in jute.