Genetic characterization of selected genera of family Rhamnaceae based on rps 11 gene

摘要

Rhamnaceae family is geographically distributed all over the world; its representation both in tropical and subtropical regions presents difficulties in tracing out its history of origin. Rhamnaceae contains 58 genera enclosing a total of 984 species. In the present study, eleven species ( Z. mauritiana , Z. spina- crhristi , Z. jujuba , Z. mauritiana var. spontanea , Z. oxyphylla , Z. nummularia , S. thea , S. filiformis , R.pentapomica , R. purpurea and H . lanceolatus ) representing different genera of family Rhamnaceae were investigated for the assessment of genetic variability. Genomic DNA from all the selected species was isolated, used as template for the amplification of rps 11 gene by a set of rps 11 primers and amplified fragments was sequenced. Sequence data was analyzed by molecular evolutionary genetics analysis (MEGA 6). It was observed that S. thea and S. filiformis in cluster I sharing same genus exhibited 99 % bootstrap value while Z. jujuba and H. lanceolatus of two different genera also showed 99 % homology manifesting close kinship among them. The value of evolutionary divergence was found to be in the range of 0.073-0.407 among the investigated species. Furthermore, genetic characterization by use of molecular markers would help in better understanding of diversity among plant species at different taxonomic ranks.