Bio-Friendly Synthesis of Silver Nanoparticles Using Mangrove Rhizophora stylosa Leaf Aqueous Extract and Its Antibacterial and Antioxidant Activity

摘要

Plant mediated biosynthesis of nanoparticles is going important due to simple processes and non-toxic materials utilization. Rhizophora stylosa (RS) mangrove leaf extract was successfully used as a bioreductor in the production of AgNPs by varying the concentration of silver nitrate and the amount of extract. Leaf extract is made by dissolving 10 grams of dried leaf powder in 100 ml double-distilled water (DDW). The concentration of silver nitrate was variable by 1,5, 10 mM with different amounts of leaf extract. The UV-Vis absorption spectrum of colloid in the range between 439-453 nm confirmed that AgNPs have been successfully synthesized. FT-IR absorption band shows the feasible biomolecules of Rhizophora stylosa responsible for the production of silver nanoparticles were amine, alcohol, phenol, alkyl halide, and aromatic combinations groups. The XRD pattern regulates that the synthesized AgNPs were in a face-centered cubic (fcc) crystal structure with an average size of 25 nm. TEM images informed that the synthesized AgNPs have a spherical shape with a size range between 9 to 57 nm. The average size of the nanoparticles was 30 nm. The solution of stable silver nanoparticle colloid from 1 until 3-month incubation. AgNPs have good antibacterial and antioxidant activity compared to pure plant extract.