The aim of this study was to evaluate the antimicrobial activity of six different propolis samples on different groups of microorganisms and compare between their chemical compositions. The antimicrobial activities of ethanolic propolis extracts were tested against four Gram-positive bacteria {Bacillus cereus, Clostridium perfringens, Listeria monocytogenes, Staphylococcus aureus), six Gramnegative bacteria (Escherichia pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella sonnei, enterocolitica), two yeasts albicans,Saccharomyces cerevisiae)and mold (Aspergillus niger) by disc diffusion and microdilution method. Minimum inhibitory concentration (MIC) was determinated using serial dilution technique to determine the antibacterial potency. The MIC values against these bacteria ranged from 6.25 to 50 ^g/mL. Bioactive components of ethanolic propolissamples were determined by high-temperature high-resolution gas chromatography coupled to mass spectrometry. According to this study ethanolic propolis samples can be used as alternative for the current synthetic antimicrobial and antioxidant agents.
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机译:本研究的目的是评估六种不同的蜂胶样品在不同的微生物组上的抗菌活性,并在其化学组合物之间进行比较。测试乙醇蜂胶提取物的抗微生物活性针对四革兰氏阳性细菌(Bacillus Cereyus,Perfringens,Histeria单核细胞增生,葡萄球菌(颅粒细菌),六个革氏细菌(大肠杆菌肺炎,铜绿假单胞菌,沙门氏菌毒蕈类,志贺氏菌,Enterocolitica),两种酵母椎间盘扩散和微量稀释方法对albicans,酿酒酵母酿酒酵母)和霉菌(Aspergillus niger)。使用连续稀释技术确定最小抑制浓度(MIC)以确定抗菌性效力。对这些细菌的麦克风值范围为6.25至50 ^ g / ml。通过偶联至质谱法测定乙醇刺激物的生物活性成分。根据该研究,乙醇蜂胶样品可用作当前合成抗微生物和抗氧化剂的替代品。
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