Isolation, production, purification, assay and characterization of fibrinolytic enzymes (Nattokinase, Streptokinase and Urokinase) from bacterial sources

摘要

Nattokinase, Streptokinase and Urokinase are novel fibrinolytic enzymes which are isolated from?Bacillus subtilis,?β-haemolytic?Streptococci?and urine sample. The fibrinolytic enzyme Nattokinase, Streptokinase and Urokinase was purified from super natant of?Bacillus subtilis,?β-haemolytic?Streptococci?and recombinant?E.colicontaining short?fragment genomic DNA of?Pseudomonas?sp.???Culture broth and showed thermophilic, hydrophilic, and strong fibrino lytic activity. The optimum temperature and pH of Nattokinase, Streptokinase and Urokinase were 37-55°C and 9, 27-37°C and 7 and 55°C and 9, respectively. The molecular weight of Nattokinase, Streptokinase and Urokinase was approximately 28 kDa, 47 kDa and 34 kDa, respectively, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The?caseinolytic activity?of Nattokinase, Streptokinase and Urokinase were?576.73?U, 467.73 U and 785.73 U, respectively, while fibrinolytic activity?achieved by fibrin plate method were 10 U, 5 U and 15 U, respectively.