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Acacia Xylan as a Substitute for Commercially Available Xylan and Its Application in the Production of Xylooligosaccharides

机译:金合欢木瓜作为市售木环的替代品及其在二醇的生产中的应用

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Over the past two decades, birchwood and beechwood xylans have been used as a popular substrate for the characterization of xylanases. Recently, major companies have discontinued their commercial production. Therefore, there is a need to find an alternative to these substrates. Xylan extraction from Acacia sawdust resulted in 23.5% (w/w) yield. The extracted xylan is composed of xylose and glucuronic acid residues in a molar ratio of 6:1 with a molecular mass of ~70 kDa. The specific optical rotation analysis of extracted xylan displayed that it is composed of the d-form of xylose and glucuronic acid monomeric sugars. The nuclear magnetic resonance analysis of extracted xylan revealed that the xylan backbone is substituted with 4-O -methyl glucuronic acid at the O2 position. Fourier transform infrared analysis confirmed the absence of lignin contamination in the extracted xylan. Xylanase from Clostridium thermocellum displayed the enzyme activity of 1761 U/mg against extracted xylan, and the corresponding activity against beechwood xylan was 1556 U/mg, which confirmed that the extracted xylan could be used as an alternative substrate for the characterization of xylanases.
机译:在过去的二十年中,Birchwood和Beechwood Xylans已被用作用于木聚糖酶的鉴别的流行底物。最近,主要公司已停产其商业生产。因此,需要找到这些基板的替代品。来自金合欢锯末的木聚糖引发为23.5%(w / w)产量。萃取的木聚糖由木糖和葡糖醛酸残基组成,摩尔比为6:1,分子量为〜70kDa。提取的木聚糖的特定光学旋转分析显示它由木糖和葡糖醛酸单体糖的D形组成。提取的木环的核磁共振分析显示,木聚糖骨架在O 2位置被4- O-甲基葡糖醛酸被取代。傅里叶变换红外分析证实了在提取的木聚糖中没有木质素污染。来自梭菌热团块的木聚糖酶显示出1761u / mg的酶活性,对萃取的Xylan酶活性,并且对山茱萸木聚糖的相应活性为1556u / mg,这证实萃取的木聚糖可以用作表征的替代基板木聚糖酶。

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