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Quantitative analysis of differentially expressed proteins in psoriasis vulgaris using tandem mass tags and parallel reaction monitoring

机译:使用串联质量标签和平行反应监测定量分析牛皮癣常见表达蛋白质

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Psoriasis vulgaris (PV) is a chronic autoimmune inflammatory disease with epidermal hyperkeratosis and parakeratosis. The study was to elucidate the pathogenesis of PV by quantitative proteomic?analysis of skin?lesion biopsies of PV and healthy tissues with tandem mass tags (TMTs) coupled?with liquid chromatography–mass spectrometry (LC–MS)/MS. A total of 4562 differentially expressed proteins (DEPs) between PV lesional tissues (n?=?11) and healthy tissues (n?=?11) were identified, of which 299 were upregulated and 206 were downregulated using fold change ??1.3 as the cutoff threshold. The Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment?analysis revealed that the DEPs were mainly enriched in the activation of immune cells (drug metabolism pathway, NOD-like pathway, and IL-17 pathway), cell proliferation (ribosomal pathway, DNA replication pathway, and base replication pathway), metabolism-related pathways (fatty acid biosynthesis and metabolism, PPAR pathway, glycerophospholipid metabolism, and cortisol synthesis and breakdown), and glandular secretion (saliva secretion, gastric acid secretion, and pancreatic fluid secretion). Thirteen DEPs that were relatively highly expressed in the drug metabolism pathway were validated with parallel reaction monitoring (PRM), of which MPO, TYMP, IMPDH2, GSTM4, and ALDH3A1 were highly expressed in PV, whereas CES1, MAOB, MGST1, and GSTT1 were less expressed in PV. These findings confirmed that these proteins participate in the drug metabolism-other enzyme pathways and play crucial roles in the activation and proliferation of immune cells in the pathogenesis of PV.
机译:牛皮癣寻常症(PV)是一种慢性自身免疫性炎症性炎症,具有表皮型高表带和平衡症。该研究是通过定量蛋白质组阐明PV的发病机制?皮肤的分析?PV和健康组织的病变活组织检查,串联质量标签(TMT)偶联?液相色谱 - 质谱(LC-MS)/ MS。鉴定了PV损伤组织(N =α11)和健康组织(N =α11)之间的4562次差异表达的蛋白质(DEP),其中有299次上调,并且使用折叠变化下调206例?>?1.3作为截止阈值。基因本体(GO)注释和基因和基因组(KEGG)途径富集的kyoto百科全书富集?分析显示,DEPS主要富集免疫细胞(药物代谢途径,点状途径和IL-17途径)的激活,细胞增殖(核糖体途径,DNA复制途径和基础复制途径),新陈代谢相关途径(脂肪酸生物合成和代谢,PPAR途径,甘油磷脂代谢和皮质醇合成和崩溃),以及腺分泌(唾液分泌,胃酸分泌和胰液流体分泌)。在药物代谢途径中相对高度表达的十六型DEP与平行反应监测(PRM)验证,其中MPO,TYMP,IMPDH2,GSTM4和ALDH3A1在PV中高度表达,而CES1,MAOB,MGST1和​​GSTT1是高度表达的在光伏中表达较少。这些发现证实,这些蛋白质参与了药物代谢 - 其他酶途径,并在PV的发病机制中的免疫细胞的活化和增殖中起至关重要的作用。

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