Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe

摘要

Peroxynitrite (ONOO ~(?) ) is a powerful and short-lived oxidant formed in vivo , which can react with most biomolecules directly. To fully understand the roles of ONOO ~(?) in cell biology, improved methods for the selective detection and real-time analysis of ONOO ~(?) are needed. We present a water-soluble, luminescent europium( III ) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe to measure ONOO ~(?) in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu( III ) probe, we monitored the production of ONOO ~(?) in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment.