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Time-resolved fluorimetric detection of lanthanide labeled nucleotides
Time-resolved fluorimetric detection of lanthanide labeled nucleotides
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机译:时间分辨荧光检测镧系元素标记的核苷酸
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摘要
Nucleotides conjugated with a chelating agent and labeled with a lanthanide, especially terbium, are readily detected by time-resolved fluorimetry during and following gel electrophoresis. The labeled nucleotide conjugates are very stable, highly fluorescent with a long radiative lifetime and remain fluorescent during dilution and during gel electrophoresis and since an enhancement solution is not required for detection, the labeled nucleotide conjugates can be detectd on-line, if desired.
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