Time-resolved fluorescence spectrometric detection method of lanthanide labeled nucleotide
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机译:镧系标记核苷酸的时间分辨荧光光谱检测方法
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摘要
Nucleotides conjugated with a chelating agent and labeled with a lanthanide, especially terbium, are readily detected by time-resolved fluorimetry during and following gel electrophoresis. The labeled nucleotide conjugates are very stable, highly fluorescent with a long radiative lifetime and remain fluorescent during dilution and during gel electrophoresis and since an enhancement solution is not required for detection, the labeled nucleotide conjugates can be detected on-line, if desired.
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