PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3

摘要

Many positive-strand RNA viruses remodel theendomembrane to form specialized replication organelles.However, knowledge regarding whethernegative-strand RNA viruses take advantage of intracellularmembranes for replication is limited. Here weshow that a negative-strand RNA virus, human parainfluenzavirus type 3 (HPIV3), remodels the endoplasmicreticulum (ER) membrane to form inclusionbodies (IBs), whereby the phosphoprotein (P) ofHPIV3 recruits phosphatidylinositol 4-kinase beta(PI4KB) to IBs to generate PI4P, creating a PI4P-enrichedmicroenvironment to promote HPIV3 replication.In addition, we find that human respiratorysyncytial virus (HRSV) also takes advantage of theER to form IBs and that these IBs are also enrichedwith PI4P. The nucleoprotein of HRSV recruitsPI4KB to IBs. These results suggest that paramyxovirusesalso exploit the host endomembrane toform IBs and that PI4KB is recruited by viral proteinsto enrich IBs with PI4P to facilitate viral replication.