Study on Influence of Apitherapy in Acrylamide Experimentally Induced Liver Disease Regarding the Hepatic Enzyme Profile in Wistar Rats

摘要

Introduction. The purpose of this experiment was to study the influence ofapitherapy in acrylamide-induced liver disease in rats. The experimental study objectiveswere the evaluation of liver enzyme parameters (ALT, TGO, AP, GGT), in conditions ofchronic liver damage induced by acrylamide. To reduce the factors that accelerate theprogress of liver damage we have administered Apitherapy products.Materials and methods. Materials: Apitherapy products Apiregya, ApiImunomod,ApiImunostim. The Apitherapy products were purchased from "Stupina LLC. The animalswere handled under thiopental induced general anesthesia. Determining the level ofinvestigated parameters, i.e. serum total protein was measured using an automatic analyzer(Aeroset, Abbott) and commercial kits (Abbott, USA). A total of 40 Wistar white rats wereused in the study; they were divided evenly into four groups: standard diet control group(group I), apitherapy and royal jelly control group (group II), acrylamide group (group III),acrylamide-apitherapy- royal jelly control group (group IV). The animals were kept understandard light and temperature conditions with access to food and water ad libitum. The toxicliver disease in rats was experimentally induced by gastric gavage administration ofacrylamide (in aqueous solution, 50 mg /kg body weight).Results and discussions. Administration of apitherapy and royal jelly (RJ) productsin experimental animals with acrylamideinduced hepatopathy (Group IV) produced areduction towards normal limits of liver enzymes compared to the other studied groups, asfollows: i). compared to the control group with standard diet (Group I), for ALT (93.85 ?2.26 vs. 110.66 ? 10.52); AST (64.78 ? 1.47 vs. 57.94 ? 9.1), alkaline phosphatase (112.42 ?4.99 vs. 86.44 ? 6.93), GGT (0.571 ? 0.07 vs. 0.233 ? 0.11); ii) compared to the grouptreated with apitherapy and RJ (Group IV), for: ALT (55.28 ? 1.49 vs. 110.66 ? 10.52); AST(35.35 ? 0.71 vs. 57.96 ? 9.1), alkaline phosphatase (97.28 ? 1.38 vs. 86.44 ? 6.93), GGT(0.457 ? 0.05 vs. 0.233 ? 0.11). The administration of the apitherapy + RJ to the group whichreceived the first the acrylamide (group IV) was effective for maintaining the TGO valueswithin close limits to the standard food group values (group II); bringing within the absolutenormal limits for TGP value and bringing within limits comparable with the values of groupII, which received the apitherapy and royal jelly.Conclusions. It is recommended to administer the Apitherapy preparations Apiregya,ApiImunostim, ApiImunomod, for normalizing the liver enzymes in liver damage induced bytoxic food.Acknowledgments. This paper was financially supported by the European Social Fund and theRomanian Government under project PERFORM-ERA (ID-57649).