Enzymatic and Mutational Analyses of a Class II 3′,5′-Cyclic Nucleotide Phosphodiesterase, PdeE, from Myxococcus xanthus

摘要

Myxococcus xanthus PdeE, an enzyme homologous to class II 3′,5′-cyclic nucleotide phosphodiesterases, hydrolyzed cyclic AMP (cAMP) and cGMP with K_m values of 12 μM and 25 μM, respectively. A pdeE mutant exhibited delays in fruiting body and spore formation compared with the wild type when cultured on starvation medium.