Impact of Hepatitis C Virus (HCV) Genotypes on Quantification of HCV RNA in Serum by COBAS AmpliPrep/COBAS TaqMan HCV Test, Abbott Antiretroviral Therapy HCV RealTime Assay, and VERSANT HCV RNA Assay

Edouard Tuaillon; Anne-Marie Mondain; Laure Ottomani; Laurent Roudière; Pascal Perney; Marie-Christine Picot; Fabienne Séguret; Fran?ois Blanc; Dominique Larrey; Philippe Van de Perre; Jacques Ducos

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Impact of Hepatitis C Virus (HCV) Genotypes on Quantification of HCV RNA in Serum by COBAS AmpliPrep/COBAS TaqMan HCV Test, Abbott Antiretroviral Therapy HCV RealTime Assay, and VERSANT HCV RNA Assay

机译：丙型肝炎病毒（HCV）基因型对COBAS AmpliPrep / COBAS TaqMan HCV测试，雅培抗逆转录病毒疗法HCV实时测定和VERSANT HCV RNA测定对血清中HCV RNA定量的影响

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The VERSANT HCV RNA 3.0 (bDNA), COBAS AmpliPrep/COBAS TaqMan HCV, and Abbott ART HCV RealTime assays were compared for hepatitis C virus RNA quantification in 158 clinical specimens (genotypes 1 to 5). RNA values differed significantly between methods (P < 0.0001), and mean titer differences ranged from 0.01 to 0.50 log₁₀ IU/ml depending on the genotypes.

机译：在158个临床样本（基因型1-5）中比较了VERSANT HCV RNA 3.0（bDNA），COBAS AmpliPrep / COBAS TaqMan HCV和Abbott ART HCV RealTime测定法对丙型肝炎病毒RNA定量的影响。方法之间的RNA值差异显着（ P <0.0001），平均滴度差异取决于基因型，范围在0.01至0.50 log _{10 IU / ml。} 展开▼

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《Journal of Clinical Microbiology》 |2007年第9期|共5页

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Edouard Tuaillon; Anne-Marie Mondain; Laure Ottomani; Laurent Roudière; Pascal Perney; Marie-Christine Picot; Fabienne Séguret; Fran?ois Blanc; Dominique Larrey; Philippe Van de Perre; Jacques Ducos;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. Differences between Two Real-Time PCR-Based Hepatitis C Virus (HCV) Assays (RealTime HCV and Cobas AmpliPrep/Cobas TaqMan) and One Signal Amplification Assay (Versant HCV RNA 3.0) for RNA Detection and Quantification [J] . Johannes Vermehren, Annika Kau, Barbara C. G?rtner, Journal of Clinical Microbiology . 2008,第12期

机译：两种基于实时PCR的丙型肝炎病毒（HCV）测定（RealTime HCV和Cobas AmpliPrep / Cobas TaqMan）和一种用于RNA检测和定量的信号放大测定（Versant HCV RNA 3.0）之间的差异

2. Comparison of Qualitative (COBAS AMPLICOR HCV 2.0 versus VERSANT HCV RNA) and Quantitative (COBAS AMPLICOR HCV Monitor 2.0 versus VERSANT HCV RNA 3.0) Assays for Hepatitis C Virus (HCV) RNA Detection and Quantification: Impact on Diagnosis and Treatment of HCV Infections [J] . Isabelle Desombere, Hans Van Vlierberghe, Sibyl Couvent, Journal of Clinical Microbiology . 2005,第6期

机译：丙型肝炎病毒（HCV）RNA检测和定量的定性（COBAS AMPLICOR HCV 2.0与VERSANT HCV RNA）和定量（COBAS AMPLICOR HCV Monitor 2.0与VERSANT HCV RNA）比较：对HCV感染的诊断和治疗的影响

3. Abbott RealTime Hepatitis C Virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV Assays for Prediction of Sustained Virological Response to Pegylated Interferon and Ribavirin in Chronic Hepatitis C Patients [J] . Kentaro Matsuura, Yasuhito Tanaka, Izumi Hasegawa, Journal of Clinical Microbiology . 2009,第2期

机译：雅培实时丙型肝炎病毒（HCV）和Roche Cobas AmpliPrep / Cobas TaqMan HCV测定法可预测慢性丙型肝炎患者对聚乙二醇化干扰素和利巴韦林持续的病毒学应答

4. Simultaneous use of one extract of HCV RNA for LCx HCV RNA Quantitative and Versant HCV Genotype assays [C] . I. Menke-Mollers, M. Steinmetz, A. Meier, European Congress of Virology . 2004

机译：同时使用HCV RNA的一个提取物，用于LCX HCV RNA定量和Versant HCV基因型测定

5. Impact of highly active antiretroviral therapy on hepatitis C virus RNA levels over one year in HIV-HCV co-infected individuals. [D] . Cooper, Curtis. 2002

机译：在HIV-HCV合并感染的个体中，高活性抗逆转录病毒疗法对丙型肝炎病毒RNA水平的影响超过一年。

6. Impact of Hepatitis C Virus (HCV) Genotypes on Quantification of HCV RNA in Serum by COBAS AmpliPrep/COBAS TaqMan HCV Test Abbott Antiretroviral Therapy HCV RealTime Assay and VERSANT HCV RNA Assay [O] . Edouard Tuaillon, Anne-Marie Mondain, Laure Ottomani, 2007

机译：丙型肝炎病毒（HCV）基因型对COBAS AmpliPrep / COBAS TaqMan HCV测试雅培抗逆转录病毒疗法HCV实时测定和VERSANT HCV RNA测定对血清中HCV RNA定量的影响

7. Impact of Hepatitis C Virus (HCV) Genotypes on Quantification of HCV RNA in Serum by COBAS AmpliPrep/COBAS TaqMan HCV Test, Abbott Antiretroviral Therapy HCV RealTime Assay, and VERSANT HCV RNA Assay▿ [O] . Tuaillon, Edouard, Mondain, Anne-Marie, Ottomani, Laure, 2007

机译：丙型肝炎病毒（HCV）基因型对COBAS AmpliPrep / COBAS TaqMan HCV检测，雅培抗逆转录病毒疗法HCV实时测定和VERSANT HCV RNA测定对血清中HCV RNA定量的影响▿

8. Seminario di Aggiornamento. Epatite da Virus HCV e Nuovi Virus Potenzialmenta Epatitici: Diagnosi, Epidemiologia, Prevenzione e Terapia. VI. Istituto Superiore di Sanita, Roma, 27-28 Novembre 2003. (Updating Seminar. HCV Hepatitis Virus and New Putative V [R] . Rapicetta, M. 2003

机译：seminario di aggiornamento。 Epatite da Virus HCV e Nuovi Virus potenzialmenta Epatitici：Diagnosi，Epidemiologia，prevenzione e Terapia。 VI。 Istituto superiore di sanita，Roma，27-28 Novembre 2003.（更新研讨会.HCV肝炎病毒和新推出的V病毒

1. 高效抗逆转录病毒疗法对艾滋病合并 HCV感染儿童肝功能与病毒载量的影响 [J] . 饶和平 ,王炜 ,姚水洪 . 山东医药 . 2014,第041期

2. 丙型肝炎病毒核心抗原在HBV/HCV合并感染者血清中的检测状况及影响因素分析 [J] . 曹红 ,张卡 ,许镇 . 中山大学学报（医学科学版） . 2011,第005期

3. 用分级定量RT—PCR测定中国人血清中HCV RNA [J] . 郭晏海 ,闫小君 ,侯玉 . 世界今日医学杂志 . 2000,第001期

1. Genetically engineered hepatitis c virus (hcv) nucleic acid clone, plasmid clone that houses a life-size hcv cdna, dna or rna transcribed from the full-length hcv cdna, transfected and in vitro infected host cell line with hcv, non-human animal infected with hcv, hcv viral particle, in vitro test system, hcv cell free, hcv vaccine and hcv test set and processes for identifying a cell line that is permissive and an animal that is permissive for hcv infection, to select hcv with adaptive mutations that allow higher levels of hcv replication in a permissive cell line, to infect an animal with hcv, to spread hcv in vitro, to transduce an animal susceptible to hcv infection with a heterologous gene, to produce hcv viral particles, hcv particle proteins and hcv antibodies, to separate agents capable of to modulate hcv replication, to prepare a hcv nucleic acid and to detect hcv antibodies in a biological sample from a patient. [P] . 外国专利： BR9808147A . 2000-03-28

机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。

2. MICROSPHERE COMPOSITION OF SUSPENSION ARRAY FOR ANALYZING HEPATITIS C VIRUS (HCV) GENOTYPE AND DETECTING METHOD THEREOF TO ANALYZE HEPATITIS C VIRUS (HCV) GENOTYPE AND HCV LA TO 4A GENOTYPES [P] . 外国专利： KR20040083940A . 2004-10-06

机译：肝癌病毒HCV基因型分析的悬浮阵列的微球组成及其检测方法，以分析肝炎病毒HCV LA和HCV LA基因型为4A基因型

3. HCV RELATED OLIGONUCLEOTIDES AND DETERMINATION SYSTEM OF HCV GENOTYPES, METHODS OF DETERMINING THE HCV GENOTYPE OF A HCV STRAIN, METHODS FOR DETECTING HCV IN A SAMPLE, AND TEST KITS FOR DETERMINING HCV GENOTYPE OR FOR DETECTING HCV [P] . 外国专利： CA2125727A1 . 1994-12-15

机译：HCV相关寡核苷酸和HCV基因型测定系统，测定HCV菌株HCV基因型的方法，样品中HCV的检测方法以及测定HCV基因型或HCV的检测试剂盒

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Impact of Hepatitis C Virus (HCV) Genotypes on Quantification of HCV RNA in Serum by COBAS AmpliPrep/COBAS TaqMan HCV Test, Abbott Antiretroviral Therapy HCV RealTime Assay, and VERSANT HCV RNA Assay

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