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An enhancer trap in the ascidian Ciona intestinalis identifies enhancers of its Musashi orthologous gene

机译:海鞘Ciona intestinalis中的增强子陷阱可鉴定出其武藏直系同源基因的增强子

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摘要

Theenhancertraptechnique,establishedinemDrosophilamelanogaster/em,isaverysophisticatedtool.Despiteitsusefulness,however,therehavebeenveryfewreportsonenhancertrapsinotheranimals.TheascidianemCionaintestinalis/em,asplendidexperimentalsystemfordevelopmentalbiology,providesgoodmaterialfordevelopmentalgenetics.Recently,germlinetransgenesisofemC.intestinalis/emhasbeenachievedusingtheTc1/emmariner/emsuperfamilytransposonemMinos/em.Duringthecourseofthatstudy,oneemMinos/eminsertionlinethatshowedadifferentGFPexpressionpatternfromotherlineswasisolated.Onefascinatingpossibilityisthatanenhancertrapeventoccurredinthisline.HereweshowthataemMinos/eminsertionintheemCi-Musashi/emgenewasresponsibleforthealteredGFPexpression.emCi-Musashi/emshowedasimilarexpressionpatterntoGFP.Inaddition,intronsofemCi-Musashi/emhaveenhanceractivitythatcanaltertheexpressionpatternofnearbygenestoresemblethatofGFPinthisline.TheseresultsclearlydemonstratethatanenhancertrapeventthatentrappedenhancersofemCi-Musashi/emoccurredinemC.intestinalis/em./p/div
机译:Theenhancertraptechnique,establishedin 果蝇,isaverysophisticatedtool.Despiteitsusefulness,但是,therehavebeenveryfewreportsonenhancertrapsinotheranimals.Theascidian Cionaintestinalis ,asplendidexperimentalsystemfordevelopmentalbiology,providesgoodmaterialfordevelopmentalgenetics.Recently,germlinetransgenesisof C.intestinalis hasbeenachievedusingtheTc1 / <在研究过程中,一个 Minos 插入线被隔离,该插入线显示了其他路线上不同的GFP表达模式。一个令人着迷的可能性是hatanenhantrtrapeventocceshinthishaisheer> in>基因负责改变GFP的表达。 Ci-Musashi 表现出与GFP类似的表达模式。 Ci-Musashi 的pedenhancerss发生在 C.intestinalis

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