Trophoblast stem cells differentiate in vitro into invasive trophoblast giant cells

Myriam Hemberger; Martha Hughes; James C Cross

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Trophoblast stem cells differentiate in vitro into invasive trophoblast giant cells

机译：滋养层干细胞在体外分化为侵入性滋养层巨细胞

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Trophoblastcellsarecharacterizedbyaninvasivebehaviorintothesurroundinguterinetissue.Inrodents,anearlyperi-/endovasculartypeofinvasionexertedbytrophoblastgiantcellscanbedistinguishedfromalateinterstitialtypecarriedoutbyglycogentrophoblastcells.Analysisofthemolecularmechanismsoftrophoblastinvasionhasbeenhampered,however,bythecomplextemporalandspatialpatternsofinvasion.Weutilizedtrophoblaststem(TS)celllinestostudytrophoblastinvasioninvitroandtoestablishamodelthatfacilitatesinvestigationofthisprocessonthemolecularlevel.OurresultsshowedthattrophoblastgiantcellsthatdifferentiatefromTScellsinvitroarecapableofpenetratingareconstitutedbasementmembranematrix.Consequently,invasionrateswereincreasedinvariousgiantcelldifferentiation-promotingconditions.WealsoderivedTScelllinesthatarehomozygousforamutationoftheemHand1/emtranscriptionfactor.TheemHand1/emsupminus;/minus;/supTScellsshowedreducedlevelsofgiantcelldifferentiationandexhibitedanapproximately50%decreaseininvasionrates.Insummary,trophoblastgiantcellsthatdifferentiatefromTScellsinvitrorecapitulatetheinvasivecapacityofnormaltrophoblastcellsinvivo.TheTScellsystemisavaluabletooltoidentifyandquantitativelystudyregulatorsoftrophoblastinvasion./p/div

机译：Trophoblastcellsarecharacterizedbyaninvasivebehaviorintothesurroundinguterinetissue.Inrodents，anearlyperi- / endovasculartypeofinvasionexertedbytrophoblastgiantcellscanbedistinguishedfromalateinterstitialtypecarriedoutbyglycogentrophoblastcells.Analysisofthemolecularmechanismsoftrophoblastinvasionhasbeenhampered然而，bythecomplextemporalandspatialpatternsofinvasion.Weutilizedtrophoblaststem（TS）celllinestostudytrophoblastinvasioninvitroandtoestablishamodelthatfacilitatesinvestigationofthisprocessonthemolecularlevel.OurresultsshowedthattrophoblastgiantcellsthatdifferentiatefromTScellsinvitroarecapableofpenetratingareconstitutedbasementmembranematrix.Consequently，invasionrateswereincreasedinvariousgiantcelldifferentiation-promotingconditions.WealsoderivedTScelllinesthatarehomozygousforamutationofthe HAND1 transcriptionfactor.The HAND1 < sup> mins; / minus; TS细胞显示出巨大的细胞分化水平降低，入侵率降低了约50％。体外分化为TS细胞的rophoblastgiant细胞概括了正常滋养层细胞的体内侵袭能力。 展开▼

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《Developmental biology》 |2004年第2期|共页

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Myriam Hemberger; Martha Hughes; James C Cross;
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1. Trophoblast stem cells differentiate in vitro into invasive trophoblast giant cells. [J] . Hemberger M, Hughes M, Cross JC Developmental biology . 2004,第2期

机译：滋养层干细胞在体外分化为侵入性滋养层巨细胞。

2. Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis [J] . Marina Polei, Juliane Günther, Dirk Koczan, BMC Cell Biology . 2020,第1期

机译：牛胎盘中的滋养细胞细胞分化：无核滋养细胞和滋养细胞之间的差异表达基因涉及细胞外基质和O-聚糖生物合成的组成和重塑

3. PTHrP promotes murine secondary trophoblast giant cell differentiation through induction of endocycle, upregulation of giant-cell-promoting transcription factors and suppression of other trophoblast cell types. [J] . El Hashash AH, Kimber SJ, Esbrit P Differentiation: The Journal of the International Society of Differentiation . 2005,第4期

机译：PTHrP通过诱导内循环，上调促进巨细胞的转录因子和抑制其他滋养层细胞类型来促进鼠类继发滋养层巨细胞分化。

4. Regulation of the Primary Human Trophoblast Cell Secretome by Mechanistic Target of Rapamycin (mTOR) Signaling [C] . Susan T. Weintraub, Frederick Rosario, Sam Pardo, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：雷帕霉素（MTOR）信号传导机械靶标调节初级人滋养细胞沉淀

5. The role of murine fizzy-related protein in the trophoblast giant cell endocycle. [D] . Gabor, Jonathan Yechiel. 2001

机译：鼠泡沫相关蛋白在滋养层巨细胞内循环中的作用。

6. Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis [O] . Marina Polei, Juliane Günther, Dirk Koczan, 2020

机译：牛胎盘中的滋养层细胞分化：单核滋养层细胞和滋养层巨细胞之间差异表达的基因参与细胞外基质的组成和重塑以及O-聚糖的生物合成

7. Trophoblast differentiation in vitro: Establishment and characterisation of a serum-free culture model for murine secondary trophoblast giant cells [O] . El-Hashash, A. H K, Kimber, Sue J. 2004

机译：体外滋养层分化：小鼠继发滋养层巨细胞无血清培养模型的建立和表征

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5. 细胞表面半乳糖基转移酶介导外胎盘锥扩展和次生滋养层巨细胞迁移的证据 [J] . 张春雨 . 生殖医学杂志 . 1997,第002期

6. 牛滋养层干细胞的培养与鉴定 [C] . 黄翔华 ,韩雪洁 ,乌云毕力格 . 第七次全国动物生物技术学术研讨会暨新疆畜牧科学院第六次学术年会 . 2013

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1. p110δ及其抗体在特异性标记滋养层巨细胞（pTGC）中的应用 [P] . 中国专利： CN104155440B . 2016.02.10

2. p110δ及其抗体在特异性标记滋养层巨细胞(pTGC)中的应用 [P] . 中国专利： CN104155440A . 2014-11-19

3. Polynucleotides and Polypeptides Associated with Trophoblast Cell Death, Differentiation, Invasion and/or Cell Fusion and Turnover [P] . 外国专利： US2014038908A1 . 2014-02-06

机译：与滋养层细胞死亡，分化，侵袭和/或细胞融合和周转相关的多核苷酸和多肽

4. DIAGNOSTIC COMPOSITION AND TREATMENT METHOD FOR CONDITION INVOLVING TROPHOBLAST CELL DEATH, DIFFERENTIATION, INVASION AND/OR CELL FUSION AND TURNOVER [P] . 外国专利： JP2010185878A . 2010-08-26

机译：涉及滋养细胞细胞死亡，分化，侵袭和/或细胞融合和周转的疾病的诊断组成和治疗方法

5. DIAGNOSTIC COMPOSITIONS AND TREATMENT METHODS FOR CONDITIONS INVOLVING TROPHOBLAST CELL DEATH, DIFFERENTIATION, INVASION AND/OR CELL FUSION AND TURNOVER [P] . 外国专利： US2009246773A1 . 2009-10-01

机译：涉及滋养细胞死亡，分化，浸润和/或细胞融合和周转的疾病的诊断组成和治疗方法

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Trophoblast stem cells differentiate in vitro into invasive trophoblast giant cells

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