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Studies on human uterine cervix and rat uterus using S-, X- and Q-band electron-spin-resonance spectroscopy

机译:S,X和Q波段电子自旋共振光谱法研究人子宫和大鼠子宫

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pIn previous studies we have reported on the detection of a strong e.s.r. signal in samples of normal human cervix; the signal is much reduced or absent in samples of invasive cancer of the cervix. In order to identify the species responsible for the strong signal, we have used X-, S- and Q-band e.s.r. spectroscopy. The major signal that is detectable in ground-up samples of cervix preserved at ?196 degrees C has features consistent with the presence of a peroxy free radical. Good agreement with the experimental findings was obtained by computer simulation, using values for the g-tensor of gx = 2.002, gy = 2.005 and gz = 2.036. The peroxy radical is produced on grinding the normal cervix samples to a powder under liquid N2, and appears to be formed by modification of a pre-existing oxygen-containing complex. Control experiments eliminated the possibility that the strong signals seen in frozen powders prepared from normal cervix were artefacts only of the grinding procedure. Experiments with rats in vivo and with cervix samples in vitro are consistent with the conclusion that the peroxy radical is formed by disturbing the cyclo-oxygenase system that is involved in prostaglandin synthesis./p
机译:>在先前的研究中,我们报告了检测到强e.s.r.正常人宫颈样本中的信号;在宫颈浸润癌样品中,该信号大大降低或消失。为了识别造成强信号的物种,我们使用了X波段,S波段和Q波段的e.s.r.光谱学。在≤196摄氏度保存的子宫颈残留样品中可检测到的主要信号具有与过氧自由基存在一致的特征。通过计算机模拟,使用gx = 2.002,gy = 2.005和gz = 2.036的g张量的值,与实验结果取得了很好的一致性。过氧自由基是在液体N2下将正常子宫颈样品研磨成粉末时产生的,并且似乎是通过修饰预先存在的含氧络合物而形成的。对照实验消除了从正常子宫颈制备的冷冻粉末中看到的强信号仅是研磨过程的假象的可能性。在大鼠体内和子宫颈体外实验表明,过氧自由基是通过干扰前列腺素合成过程中的环加氧酶系统形成的。

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