Optimization of Culture Conditions for Production of ?-Galactosidase by Bacillus Megaterium NM56 Isolated from Raw Milk.

摘要

ABSTRACT -galactosidase (E.C.3.2.1.23) has been widely used in dairy and pharmaceutical industries. In this study, Bacillus megaterium NM56 showing maximum production of -galactosidase was isolated from raw milk. Effects of various culture conditions, namely, incubation period, temperature, pH, carbon source and nitrogen source were investigated. Results revealed that the highest level of -galactosidase was produced after 48 h of incubation. Optimal temperature and pH for production was observe.