In-Vitro Micropropagation through Cotyledonary nodal segments in Prosopis cineraria L.

摘要

ABSTRACT A Protocol has been developed for micropropagation of a multipurpose tree of desert i.e Prosopis cineraria L under aseptic conditions. Cotyledonary nodal segments (1 to 1.5 cm) were taken from 15 to 20 days old aseptically grown seedlings. 15-20 shoot buds were induced on Murashige and Skoog (1962) medium supplemented with various cytokinins and auxins individually and in various combinations. Best response was observed in 2.0 mg/l BAP added singly and 2.0 mg/l BAP and 0.05 mgl IBA in .