Nanoparticle self‐assembly by a highly stable recombinant spider wrapping silk protein subunit

摘要

Artificialspidersilkproteinsmayformfiberswithexceptionalstrengthandelasticity.Wrappingsilk,oraciniformsilk,isthetoughestofthespidersilks,andhasaverydifferentproteincompositionthanotherspidersilks.Here,wepresentthecharacterizationofanaciniformprotein(AcSp1)subunitnamedW1,consistingofoneAcSp1199residuerepeatunitfromArgiopetrifasciata.ThestructuralintegrityofrecombinantW1isdemonstratedinavarietyofbufferconditionsandtimepoints.Furthermore,weshowthatW1hasahighthermalstabilitywithreversibledenaturationat～71°Candformsself‐assemblednanoparticleinnear‐physiologicalconditions.W1thereforerepresentsahighlystableandstructurallyrobustmoduleforprotein‐basednanoparticleformation...