Distinct tRNA modifications in the thermo‐acidophilic archaeon, Thermoplasma acidophilum

摘要

Thermoplasmaacidophilumisathermo‐acidophilicarchaeon.WepurifiedtRNALeu(UAG)fromT.acidophilumusingasolid‐phaseDNAprobemethodanddeterminedtheRNAsequenceafterdeterminingvianucleosideanalysisandm7G‐specificanilinecleavagebecauseithasbeenreportedthatT.acidophilumtRNAcontainsm7G,whichisgenerallynotfoundinarchaealtRNAs.RNAsequencingandliquidchromatography–massspectrometryrevealedthatthem7Gmodificationexistsatanovelposition49.Furthermore,wefoundseveraldistinctmodifications,whichhavenotpreviouslybeenfoundinarchaealtRNA,suchas4‐thiouridine9,archaeosine13and5‐carbamoylmethyuridine34.TherelatedtRNAmodificationenzymesandtheirgenesarediscussed...