MODIFICATION OF TRNA T-STEM FOR ENHANCING N-METHYL AMINO ACID INCORPORATION

摘要

The present invention addresses the problem of developing a method for synthesizing a novel peptide which can co-incorporate a plurality of different N-methyl amino acids and discover a highly N-methylated peptide having improved membrane-permeability and stability to proteolysis. The present invention provides tRNA that encodes N-methyl amino acids and has a T-stem in which N₁N₂GGN₃ pairs with N₄CCN₅U (N₁ is G or A, one among N₂ and N₄ is G and the other is C, and N₃ and N₄ are any pairing amino acids).