Three‐dimensional crystal structure of recombinant erabutoxin a at 2.0 Å resolution

摘要

>Recombinant erabutoxin a (Ea_r) has been crystallized by vapour diffusion in hanging drops. The crystals belong to space group P2₁2₁2₁ with cell dimensions a = 55.8 Å, b = 53.4 Å, c = 40.8 Å. Diffraction data have been recorded on a FAST detector up to 2.0 Å. The atomic crystal structure of Ea_r has been determined by initial refinement of the structure of the isotoxin erabutoxin b (Eb) the crystals of which were grown under identical conditions. The R-factor was 23% at 2.0 Å resolution. The secondary and tertiary structures of Ea_r are shown to be identical with that of wild-type Eb, within the experimental error.