Tumor necrosis factor is markedly synergistic with interleukin 1 and interferon‐γ in stimulating the production of nerve growth factor in fibroblasts

摘要

>A possible interaction between tumor necrosis factor-α (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1α (IL-1α), IL-1β and interferon-γ (IFN-γ), but was antagonized by transforming growth factor-β (TGF-β). The most remarkable synergistic effect was observed between TNF and IL-1α/β; as little as 0.003 data-equation-construct="true" class="math-equation-construct"> data-equation-image="true" class="math-equation-image"> alt="math formula" src="http://onlinelibrary.wiley.com/store/10.1016/0014-5793(94)80132-0/asset/equation/feb20014579394801320-math-si1.gif?v=1&s=7e097f9e72c5443a384e7ca2557c971b40a32682" class="inlineGraphic" /> data-equation-mathml="true" class="math-equation-mathml" style="display:none"> of IL-1β markedly enhanced TNF's stimulatory activity on NGF production in the cells. These findings reinforce the idea that TNF, in concert with IL-1α/β, plays an essential role in regulating the regeneration of peripheral nerves following injury through an indirect mechanism by which it stimulates NGF production in fibroblasts.