Luteinizing hormone‐releasing hormone (LHRH)‐induced arachidonic acid release in rat granulosa cells Role of calcium and protein kinase C

摘要

>In rat granulosa cells prelabeled with [3H]arachidonic acid, addition of LHRH, A23187 or 12-0-tetradecanoyl phorbol-13-acetate (TPA) enhanced the release of [3H]arachidonic acid into the culture medium. The effect of A23187 was significant as early as 5 min and the lowest effective dose was 5 × 10−8/M. On the other hand, TPA was effective only at dosages greater than 10−6M. These results suggest that the stimulatory effect of LHRH on arachidonic acid release is coupled more tightly to a Ca2+-dependent rather than a protein kinase C-mediated pathway.