Construction of a Quantitative Acetylomic Tissue Atlas in Rice (Oryza sativa L.)

摘要

PKA (protein lysine acetylation) is a key post-translational modification involved inthe regulation of various biological processes in rice. So far, rice acetylome data is very limiteddue to the highly-dynamic pattern of protein expression and PKA modification. In this study,we performed a comprehensive quantitative acetylome profile on four typical rice tissues, i.e.,the callus, root, leaf, and panicle, by using a mass spectrometry (MS)-based, label-free approach.The identification of 1536 acetylsites on 1454 acetylpeptides from 890 acetylproteins representedone of the largest acetylome datasets on rice. A total of 1445 peptides on 887 proteins weredifferentially acetylated, and are extensively involved in protein translation, chloroplast development,and photosynthesis, flowering and pollen fertility, and root meristem activity, indicating the importantroles of PKA in rice tissue development and functions. The current study provides an overall viewof the acetylation events in rice tissues, as well as clues to reveal the function of PKA proteins inphysiologically-relevant tissues.